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KMID : 1161420110140060625
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Journal of Medicinal Food
2011 Volume.14 No. 6 p.625 ~ p.629
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Fibrinolytic and Antiplatelet Aggregation Properties of a Recombinant Cheonggukjang Kinase
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Radnaabazar Chinzorig
Park Chung-Mu
Kim Jeong-Hwan
Cha Jae-Ho
Song Young-Sun
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Abstract
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This study characterized the efficacy of recombinant Cheonggukjang kinase (CGK) 3?5-rich fraction as a thrombolytic agent, which we overexpressed in Bacillus licheniformis ATCC10716, a strain normally lacking fibrinolytic activity. We found that CGK3-5 is a plasmin-like protease that directly degrades fibrin clots and does not activate plasminogen during fibrin clot lysis and platelet-rich clot lysis assays. We also confirmed antiplatelet and antithrombotic activity by CGK3-5-rich fraction both in vitro and in vivo. CGK3-5-rich fraction inhibited collagen-induced platelet aggregation in platelet-rich plasma in a concentration-dependent manner. The concentration of 1.5?mg/mL CGK3-5-rich fraction completely inhibited collagen-induced platelet aggregation. Furthermore, injection of CGK3-5-rich fraction into tail veins dose-dependently protected mice from death by pulmonary embolism induced by collagen and epinephrine. The survival rates were 30%, 70%, and 100%, respectively, with doses of 130?mg/kg, 260?mg/kg, and 520?mg/kg. These findings suggest that CGK3-5 holds promise as a treatment to mitigate the potentially effects of stroke and heart failure.
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KEYWORD
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antiplatelet aggregation, antithrombotic activity, Cheonggukjang kinase, plasmin-like protease
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